rabbit anti cyclin e antibody  (Bethyl)

Journal: Scientific Reports

Article Title: 6-Gingerol alleviates high glucose-induced inflammation and cytotoxicity in THP-1 cells by inhibiting TLR4 signaling

doi: 10.1038/s41598-025-34192-z

Figure Lengend Snippet: 6-Gingerol regulates cell cycle-related proteins in THP-1 cells under high-glucose conditions. ( A ) Western blot analysis of CDK4, Cyclin D1, and Cyclin E protein expression in THP-1 cells treated with glucose (0, 5.5 or 25 mM) and 6-gingerol (30 or 60 µM) for 24 h. Protein levels were normalized to β-actin and quantified by densitometry. ( B ) Quantitative real-time PCR (qPCR) analysis of CDK4 , CCND1 , and CCNE1 mRNA expression under the same treatment conditions. mRNA levels were normalized to GAPDH and expressed relative to the untreated control. ( C ) Western blot analysis of p21, p27, p53, MDM2, and phosphorylated MDM2 (p-MDM2) protein expression following 24 h of treatment. ( D ) qPCR analysis of CDKN1A , CDKN1B , and TP53 mRNA expression, normalized to GAPDH and expressed relative to control. All data represent the mean ± SEM from three independent experiments. Statistical significance was assessed by one-way ANOVA. # p < 0.001 versus control; *** p < 0.001.

Article Snippet: Primary antibodies against TLR4 (sc-293072), p-IκBα (sc-8404), and β-actin (sc-47778), along with secondary antibodies (anti-mouse (sc-516102) and anti-rabbit (sc-2357)), COX‐1 (sc‐19998), COX‐2 (sc‐19999), cyclin E (sc-481), p21 (sc-756), and CDK4 (sc-260), were acquired from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

Techniques: Western Blot, Expressing, Real-time Polymerase Chain Reaction, Control